Abbreviation for "Enzyme-Linked Immuno Sorbent Assay". This is a serological test procedure (serology = diagnosis of infectious diseases) that has been used since the mid-1970s and is now widely used for the identification of disease pathogens in vines, which is also the case for imports quarantine he follows. With the help of ELISA, the content of proteins ( proteins ) and virus but also connections like pesticides, Hormones and toxins are detected. This makes use of the property of specific antibodies that bind to the substance to be detected (antigen). Antibody or antigen are previously labeled with an enzyme.
The by the enzyme catalyzed reaction serves as evidence for the presence of the antigen. In the grapevine This is done by detecting viral protein (protein) in the plant tissue. Thus, for example, the infestation by those caused by viruses Leaf roll disease and brushwood disease be determined. Appropriately prepared test sets are used. Such tests are also used in the breeding (Conservation Breeding). For the determination of edible white, to the allergens substances in wine, only the ELISA method is authorized. Other test methods are the so-called Indexing such as PCR (Polymerase Chain Reaction). See also a disease list below Vine enemies,