Abbreviation for "Enzyme-Linked Immuno Sorbent Assay". It is a serological test procedure (serology = diagnosis of infectious diseases) that has been in use since the mid-1970s and is now widespread for the determination of pathogens in vines, which is also the case in imports quarantine he follows. With the help of ELISA, the content of proteins ( proteins ) and virus, but also connections like pesticides, Hormones and toxins are detected. Here, one takes advantage of the property of specific antibodies that bind to the substance (antigen) to be detected. Antibodies or antigens are previously labeled with an enzyme.
The through that enzyme catalyzed reaction serves as evidence of the presence of the antigen. In the grapevine this is done by detecting viral protein in the plant tissue. For example, the infestation can be caused by viruses Leaf roll disease and brushwood disease be determined. Appropriately prepared test sets are used. Such tests are also carried out at the breeding (Conservation breeding) carried out. For the determination of albuminous, to the allergens counting substances in wine is only permitted the ELISA method. Other test procedures are the so-called Indexing such as PCR (Polymerase chain reaction). See also a list of diseases below Vine enemies,